Publications

Qiao, E., Baek, J., Fulmore, C., Song, M., Kim, T.-S., Kumar, S., & Schaffer, D. v. (2024). Spectrin mediates 3D-specific matrix stress-relaxation response in neural stem cell lineage commitment. Science Advances, 10(31), 8232. https://doi.org/10.1126/SCIADV.ADK8232 View PDF
Recent Selected Publications

Sampayo, R. G., Sakamoto, M., Wang, M., Kumar, S., & Schaffer, D. V. (2023). “Mechanosensitive stem cell fate choice is instructed by dynamic fluctuations in activation of Rho GTPases.” Proceedings of the National Academy of Sciences of the United States of America, 120(22). https://doi.org/10.1073/PNAS.2219854120. View PDF

 

Recent Selected Publications

Baek, J., Kumar, S., Schaffer, D.V., & Im, S.G. (2022). “N-Cadherin adhesive ligation regulates mechanosensitive neural stem cell lineage commitment in 3D matrices”. Biomaterials Science, 10(23), 6768–6777. https://doi.org/10.1039/D2BM01349E. View PDF

Recent Selected Publications

Qiao EL, Kumar S, Schaffer DV. Mastering their own fates through the matrix. Nat Mater. 2019 Aug; 18(8):779-780. doi: 10.1038/s41563-019-0429-x. PubMed PMID: 31332317. View PDF

Recent Selected Publications

Muckom R, McFarland S, Yang C, Perea B, Gentes M, Murugappan A, Tran E, Dordick JS, Clark DS, Schaffer DV. High-throughput combinatorial screening reveals interactions between signaling molecules that regulate adult neural stem cell fate. Biotechnol Bioeng. 2019 Jan; 116(1):193-205. doi: 10.1002/bit.26815. Epub 2018 Nov 6. PubMed PMID: 30102775; PubMed Central PMCID: PMC6289657. View PDF

Recent Selected Publications

Nierode GJ, Gopal S, Kwon P, Clark DS, Schaffer DV, Dordick JS. High-throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture. Biotechnol Bioeng. 2019 Jan; 116(1):168-180. doi: 10.1002/bit.26839. Epub 2018 Oct 27. PubMed PMID: 30229860; PubMed Central PMCID: PMC6289706. View PDF

Recent Selected Publications

Sun S, Schaffer DV. Corrigendum to “Engineered viral vectors for functional interrogation, deconvolution, and manipulation of neural circuits” [Curr. Opin. Neurobiol. 50 (2018) 163-170]. Curr Opin Neurobiol. 2018 Dec; 53:220. doi: 10.1016/j.conb.2018.08.005. Epub 2018 Sep 5. PubMed PMID: 30196036. View PDF

Recent Selected Publications

Adil MM, Schaffer DV. Cheaper and less variable expansion. Nat Biomed Eng. 2018 Mar; 2(3):144-145. doi: 10.1038/s41551-018-0209-y. PubMed PMID: 31015716. View PDF

Recent Selected Publications

Adil MM, Schaffer DV. hPSC-derived Midbrain Dopaminergic Neurons Generated in a Scalable 3-D Biomaterial. Curr Protoc Stem Cell Biol. 2018 Feb 28; 44:2D.21.1-2D.21.17. doi: 10.1002/cpsc.47. PubMed PMID: 29512105. View PDF

Recent Selected Publications

Day TP, Byrne LC, Flannery JG, Schaffer DV. Screening for Neutralizing Antibodies Against Natural and Engineered AAV Capsids in Nonhuman Primate Retinas. Methods Mol Biol. 2018; 1715:239-249. doi: 10.1007/978-1-4939-7522-8_17. PubMed PMID: 29188518. View PDF

Recent Selected Publications

Halperin SO, Tou CJ, Wong EB, Modavi C, Schaffer DV, Dueber JE. CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window. Nature. 2018 Aug; 560(7717):248-252. doi: 10.1038/s41586-018-0384-8. Epub 2018 Aug 1. PubMed PMID: 30069054. View PDF

Recent Selected Publications

Chen, S. Y.S. Na, M.E. Todhunger, S. Hsiao, A.W. Bremer, O.J. Scheideler, P.R. Bomdica, M.M. Haharbiz, Z.J. Gartner, D.V. Schaffer (2016) “Interrogating Stem Cell Fate Decisions with High-throughput Arrays of Cellular Communities.” View PDF

Recent Selected Publications

Tervo, D.G.R., B-Y. Hwang, S. Viswanathan, T. Gaj, M. Lavzin, K.D. Ritola, S. Lindo, S. Michael, E.P. Kuleshova, D. Ojala, C-C. Huang, C.R. Gerfen, J. Schiller, J.T. Dudman, A.W. Hantman, L.L. Looger, D.V. Schaffer (co-corresponding author), and A.Y. Karpova (2016) “A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons.” , 92:372-382 (PMID 27720486). View PDF

Recent Selected Publications

Bugaj, L.J., D. P. Spelke, C.K. Mesuda, M. Varedi, R.S. Kane, and D.V. Schaffer (2015)”Regulation of Endogenous Transmembrane Receptors Through Optogenetic Cry2 Clustering.” Nature Communications. View PDF

Recent Selected Publications

Kotterman, M.A. and D.V. Schaffer (2014) “Engineering Adeno-Associated Viruses for Clinical Gene Therapy.” Nature Reviews Genetics, 15:445-451 (PMID 24840552). View PDF

Recent Review Articles Recent Selected Publications

Shapiro, M.G., R.M. Ramirez, L.J. Sperling, G. Sun, A. Pines, D.V. Schaffer, and V.S. Bajaj (2014) “Genetically Encoded Reporters for Hyperpolarized Xenon.” , 6(7):629-34 (PMID: 24950334). View PDF

Recent Selected Publications

Hughes, A.J., D.P. Spelke, Z. Xu, C-C. Kang, D.V. Schaffer (co-corresponding author), and A.E. Herr (2014) “Single-cell Western Blotting.” , 11:749-755 (PMID 24880876). View PDF

Recent Selected Publications

Shapiro, M., P. Goodwill, A. Neogy, M. Yin, F. Foster, D.V. Schaffer, and S. Conolly (2014) “Biogenic Gas Nanostructures as Ultrasonic Molecular Reporters.” , 9:311-316 (PMID 24633522). View PDF

Recent Selected Publications

Lei, Y. and D.V. Schaffer (2013) “A Fully Defined and Scalable 3D Culture System for Human Pluripotent Stem Cell Expansion and Differentiation.” , 110:E5039-5048 (PMID 24248365). View PDF

Recent Selected Publications

Downing, T.L., J. Soto, C. Morez, T. Houssin, A. Fritz, F. Yuan, J. Chu, S. Patel, D.V. Schaffer, and S. Li (2013) “Biophysical Regulation of Epigenetic State and Cell Reprogramming.” , 12:1154-1162 (PMID 24141451). View PDF

Recent Selected Publications

Dalkara, D., L.C. Byrne, R.R. Klimczak, M. Visel, L. Yin, W.H. Merigan, J.G. Flannery, and D.V. Schaffer (2013) “In Vivo–Directed Evolution of a New Adeno-Associated Virus for Therapeutic Outer-Retinal Gene Delivery from the Vitreous.” , 5:189ra76 (PMID 23761039). View PDF

Recent Selected Publications

Conway, A., T. Vazin, D.P. Spelke, N.A. Rode, K.E. Healy, R.S. Kane, and D.V. Schaffer (2013) “Multivalent Ligands to Control Stem Cell Behaviour in Vitro and in Vivo.” , 8:831-838 (PMID 24141540). View PDF

Recent Selected Publications

Bugaj, L.J, A.T Choksi, C.K. Mesuda, R.S. Kane, and D.V. Schaffer (2013) “Optogenetic Protein Clustering and Signaling Activation in Mammalian Cells.” , 10:249-252. View PDF

Recent Selected Publications

Ashton, R.S., A. Conway, C. Pangarkar, J. Bergen, K-I. Lim, P. Shah, M. Bissell, and D.V. Schaffer (2012) “Astrocytes Regulate Adult Hippocampal Neurogenesis Through Ephrin-B Signaling.” , 15:1399-1406 (PMID: 22983209). View PDF

Recent Selected Publications

Dickinson, B.C., J. Peltier, D. Stone, D.V. Schaffer (co-corresponding author), and C.J Chang (2011) “Nox2 Redox Signaling Maintains Essential Cell Populations in the Brain.” , 7:106-112 (PMID 21186346). View PDF

Recent Selected Publications

Lim, K-I., R.R. Klimczak, J.H. Yu, and D.V. Schaffer (2010) “Specific Insertions of Zinc Finger Domains into Gag-Pol Yields Engineered Retroviral Vectors with Selective Integration Properties.” , 107:12475-12480 (PMID 20616052). View PDF

Recent Selected Publications

Excoffon, K.J., J.T. Koerber, D.D. Dickey, M. Murtha, S. Keshavjee, B.K. Kaspar, J. Zabner, and D.V. Schaffer (2009) “Directed Evolution of Adeno-Associated Virus to an Infectious Respiratory Virus.” Proceedings of the National Academy of Sciences USA, 106:3865-3870 (PMID 19237554). View PDF

Recent Selected Publications

Koerber, J.T., N. Maheshri, B.K. Kaspar, and D.V. Schaffer (2006) “Construction of Diverse Adeno-Associated Viral Libraries for Directed Evolution of Enhanced Gene Delivery Vehicles.” Nature Protocols, 1:701-706. View PDF

Recent Selected Publications

Maheshri, N., J.T. Koerber, B. Kaspar, and D.V. Schaffer (2006) “Directed Evolution of Adeno-Associated Virus Yields Enhanced Gene Delivery Vectors.” Nature Biotechnology, 24:198-204. View PDF

Recent Selected Publications

Weinberger, L.S., J.C. Burnett, J.E. Toettcher, A.P. Arkin, and D.V. Schaffer (2005) “Stochastic Gene Expression in a Lentiviral Feedback Loop: HIV-1 Tat Expression Drives Phenotypic Diversity.” Cell, 122:169-182. View PDF

Recent Selected Publications
Carneiro, A. D., & Schaffer, D. v. (2024). Engineering novel adeno-associated viruses (AAVs) for improved delivery in the nervous system. Current Opinion in Chemical Biology, 83, 102532. https://doi.org/10.1016/J.CBPA.2024.102532 View PDF
Recent Review Articles

Zhu, D., Schieferecke, A. J., Lopez, P. A., & Schaffer, D. v. (2021). “Adeno-Associated Virus Vector for Central Nervous System Gene Therapy”. Trends in Molecular Medicine, 27(6), 524–537. https://doi.org/10.1016/J.MOLMED.2021.03.010. View PDF

Recent Review Articles

Schaffer, D., & Shvartsman, S. Y. (2018). “Editorial overview: Tissue, cell and pathway engineering”. Current Opinion in Biotechnology, 52, iv–v. https://doi.org/10.1016/J.COPBIO.2018.07.004. View PDF

Recent Review Articles

Gaj., T., B.E. Epstein, and D.V. Schaffer (2016), “Genome Engineering Using Adeno-Associated Virus: Basic and Clinical Research Applications.” The American Society of Gene & Cell Therapy,1-7(PMID 26373345). View PDF

Recent Review Articles

Kotterman, M.A. and D.V. Schaffer (2014) “Engineering Adeno-Associated Viruses for Clinical Gene Therapy.” Nature Reviews Genetics, 15:445-451 (PMID 24840552). View PDF

Recent Review Articles Recent Selected Publications

Bartel, M.A., J.R. Weinstein, and D.V. Schaffer (2012) “Directed Evolution of Novel Adeno-Associated Viruses for Therapeutic Gene Delivery.” Gene Therapy (2012) 19, 19:694-700 (PMID 22402323). View PDF

Recent Review Articles

Asokan, A., D.V. Schaffer, and R.J. Samulski (2012) “The AAV Vector Toolkit: Poised at the Clinical Crossroads.” The American Society of Gene & Cell Therapy, 20:699-708 (PMID 22273577). View PDF

Recent Review Articles

Keung, A.J., S. Kumar, and D.V. Schaffer (2010) “Presentation Counts: Microenvironmental Regulation of Stem Cells by Biophysical and Material Cues.” Annu. Rev. Cell Dev. Biol. 2010. 26:533-556 (PMID 20590452). View PDF

Recent Review Articles

Vazin, T. and D.V. Schaffer (2010) “Engineering Strategies to Emulate the Stem Cell Niche Trends in Biotechnology.” Trends in Biotechnology Vol.28 No.3, 28:117-124 (PMID 20042248). Selected as the Cover Article. View PDF

Recent Review Articles

Zhu, D., Brookes, D. H., Busia, A., Carneiro, A., Fannjiang, C., Popova, G., Shin, D., Donohue, K. C., Lin, L. F., Miller, Z. M., Williams, E. R., Chang, E. F., Nowakowski, T. J., Listgarten, J., & Schaffer, D. v. (2024). Optimal trade-off control in machine learning-based library design, with application to adeno-associated virus (AAV) for gene therapy. Science Advances, 10(4). https://doi.org/10.1126/SCIADV.ADJ3786 [PDF]

Baek, J., Kumar, S., & Schaffer, D. v. (2024). Dynamic light-responsive RhoA activity regulates mechanosensitive stem cell fate decision in 3D matrices. Biomaterials Advances, 213836. https://doi.org/10.1016/J.BIOADV.2024.213836 View PDF
Qiao, E., Baek, J., Fulmore, C., Song, M., Kim, T.-S., Kumar, S., & Schaffer, D. v. (2024). Spectrin mediates 3D-specific matrix stress-relaxation response in neural stem cell lineage commitment. Science Advances, 10(31), 8232. https://doi.org/10.1126/SCIADV.ADK8232 View PDF
Recent Selected Publications
Guo, J., Lin, L. F., Oraskovich, S. v., Rivera de Jesús, J. A., Listgarten, J., & Schaffer, D. v. (2024). Computationally guided AAV engineering for enhanced gene delivery. Trends in Biochemical Sciences, 49(5), 457–469. https://doi.org/10.1016/J.TIBS.2024.03.002/ASSET/4B686283-609D-453C-82AB-ABDBED7D7108/MAIN.ASSETS/GR5.JPG View PDF
Schieferecke, A. J., Lee, H., Chen, A., Kilaru, V., Krish Williams, J., & Schaffer, D. v. (2024). Evolving membrane-associated accessory protein variants for improved adeno-associated virus production. Molecular Therapy, 32(2), 340–351. https://doi.org/10.1016/J.YMTHE.2023.12.015/ATTACHMENT/BD3CDEC6-848E-41E7-8747-2DD28BD6F60D/MMC3.PDF View PDF
Carneiro, A. D., & Schaffer, D. v. (2024). Engineering novel adeno-associated viruses (AAVs) for improved delivery in the nervous system. Current Opinion in Chemical Biology, 83, 102532. https://doi.org/10.1016/J.CBPA.2024.102532 View PDF
Recent Review Articles

Sampayo, R. G., Sakamoto, M., Wang, M., Kumar, S., & Schaffer, D. V. (2023). “Mechanosensitive stem cell fate choice is instructed by dynamic fluctuations in activation of Rho GTPases.” Proceedings of the National Academy of Sciences of the United States of America, 120(22). https://doi.org/10.1073/PNAS.2219854120. View PDF

 

Recent Selected Publications

Repina, N., Johnson, H., Bao, X., Zimmerman, J., Joy, D., Bi, S., Kane, R.,  Schaffer, D.V. (2023). “Optogenetic control of Wnt signaling models cell-intrinsic embryogenic patterning using 2D human pluripotent stem cell culture “. Development. https://doi.org/10.1242/dev.201386. View PDF

Schieferecke, A. J., Lee, H., Chen, A., Kilaru, V., Krish Williams, J., & Schaffer, D. v. (2023). Evolving membrane-associated accessory protein variants for improved adeno-associated virus production. Molecular Therapy. https://doi.org/10.1016/J.YMTHE.2023.12.015. View PDF
Mulyasasmita, W., Schaffer, D. v., Stack, R., & Chapman, R. (2023). From garages to ecosystems: the coevolution of life science incubators and accelerators. Trends in Biotechnology, 0(0). https://doi.org/10.1016/J.TIBTECH.2023.11.007. View PDF

Baek, J., Kumar, S., Schaffer, D.V., & Im, S.G. (2022). “N-Cadherin adhesive ligation regulates mechanosensitive neural stem cell lineage commitment in 3D matrices”. Biomaterials Science, 10(23), 6768–6777. https://doi.org/10.1039/D2BM01349E. View PDF

Recent Selected Publications

Miller, Z. M., Harper, C. C., Lee, H., Bischoff, A. J., Francis, M. B., Schaffer, D. V., & Williams, E. R. (2022). “Apodization Specific Fitting for Improved Resolution, Charge Measurement, and Data Analysis Speed in Charge Detection Mass Spectrometry”. Journal of the American Society for Mass Spectrometry, 33(11), 2129–2137. https://doi.org/10.1021/JASMS.2C00213/ASSET/IMAGES/LARGE/JS2C00213_0004.JPEG. View PDF

Yang, S. J., O’Donnell, J. T. del B., Giordani, F., Wang, J., Lui, A., Piekarski, D., Irrinki, A., Schaffer, D. V., & Landry, M. P. (2022). “Synaptic scale dopamine disruption in Huntington’s Disease model mice imaged with near infrared catecholamine nanosensors”. BioRxiv, 2022.09.19.508617. https://doi.org/10.1101/2022.09.19.508617. View PDF

Zhu, D., Brookes, D. H., Busia, A., Carneiro, A., Fannjiang, C., Popova, G., Shin, D., Donohue, K. C., Chang, E. F., Nowakowski, T. J., Listgarten, J., & Schaffer, D.V. (2022). “Optimal trade-off control in machine learning-based library design, with application to adeno-associated virus (AAV) for gene therapy”. BioRxiv, 2021.11.02.467003. https://doi.org/10.1101/2021.11.02.467003. View PDF

Harper, C. C., Miller, Z. M., Lee, H., Bischoff, A. J., Francis, M. B., Schaffer, D. V., & Williams, E. R. (2022). “Effects of Molecular Size on Resolution in Charge Detection Mass Spectrometry”. Analytical Chemistry, 94(33), 11703–11712. https://doi.org/10.1021/ACS.ANALCHEM.2C02572/ASSET/IMAGES/LARGE/AC2C02572_0007.JPEG. View PDF

Louis, B., Tewary, M., Bremer, A. W., Philippeos, C., Negri, V. A., Zijl, S., Gartner, Z. J., Schaffer, D. V., & Watt, F. M. (2022). “A reductionist approach to determine the effect of cell-cell contact on human epidermal stem cell differentiation”. Acta Biomaterialia, 150, 265–276. https://doi.org/10.1016/J.ACTBIO.2022.07.054. View PDF

Molina, R. S., Rix, G., Mengiste, A. A., Álvarez, B., Seo, D., Chen, H., Hurtado, J. E., Zhang, Q., García-García, J. D., Heins, Z. J., Almhjell, P. J., Arnold, F. H., Khalil, A. S., Hanson, A. D., Dueber, J. E., Schaffer, D. V. , Chen, F., Kim, S., Fernández, L. Á., … Liu, C. C. (2022). “In vivo hypermutation and continuous evolution”. Nature Reviews Methods Primers 2022 2:1, 2(1), 1–22. https://doi.org/10.1038/s43586-022-00119-5. View PDF

Baek, J., Lopez, P. A., Lee, S., Kim, T. S., Kumar, S., & Schaffer, D. V. (2022). “Egr1 is a 3D matrix–specific mediator of mechanosensitive stem cell lineage commitment”. Science Advances, 8(15), 4646. https://doi.org/10.1126/SCIADV.ABM4646/SUPPL_FILE/SCIADV.ABM4646_SM.PDF. View PDF

Qiao, E., Schaffer, D.V., & Kumar, S. (2022). “Substrate stress relaxation regulates neural stem cell fate commitment”. Biophysical Journal, 121(3), 420a. https://doi.org/10.1016/J.BPJ.2021.11.655. View PDF

Zhu, D., Johnson, H. J., Chen, J., & Schaffer, D. V. (2022). “Optogenetic Application to Investigating Cell Behavior and Neurological Disease”. Frontiers in Cellular Neuroscience, 16, 4. https://doi.org/10.3389/FNCEL.2022.811493/BIBTEX. View PDF

Öztürk, B. E., Johnson, M. E., Kleyman, M., Turunç, S., He, J., Jabalameli, S., Xi, Z., Visel, M., Dufour, V. L., Iwabe, S., Marinho, F. P., Aguirre, G. D., Sahel, J. A., Schaffer, D. v., Pfenning, A. R., Flannery, J. G., Beltran, W. A., Stauffer, W. R., & Byrne, L. C. (2021). “Scaavengr, a transcriptome-based pipeline for quantitative ranking of engineered aavs with single-cell resolution”. ELife, 10. https://doi.org/10.7554/ELIFE.64175. View PDF

Croze, R. H., Kotterman, M., Burns, C. H., Schmitt, C. E., Quezada, M., Schaffer, D., Kirn, D., & Francis, P. (2021). “Viral Vector Technologies and Strategies: Improving on Nature”. International Ophthalmology Clinics, 61(3), 59. https://doi.org/10.1097/IIO.0000000000000361. View PDF

Barnes, C. R., Lee, H., Ojala, D. S., Lewis, K. K., Limsirichai, P., & Schaffer, D. v. (2021). “Genome-wide activation screens to increase adeno-associated virus production”. Molecular Therapy – Nucleic Acids, 26, 94–103. https://doi.org/10.1016/J.OMTN.2021.06.026. View PDF

Kotterman, M., Beliakoff, G., Croze, R., Vazin, T., Schmitt, C., Szymanski, P., Leong, M., Quezada, M., Holt, J., Barglow, K., Hassanipour, M., Schaffer, D., Francis, P., & Kirn, D. (2021). “Directed Evolution of AAV Targeting Primate Retina by Intravitreal Injection Identifies R100, a Variant Demonstrating Robust Gene Delivery and Therapeutic Efficacy in Non-Human Primates”. BioRxiv, 2021.06.24.449775. https://doi.org/10.1101/2021.06.24.449775. View PDF

Zhu, D., Schieferecke, A. J., Lopez, P. A., & Schaffer, D. v. (2021). “Adeno-Associated Virus Vector for Central Nervous System Gene Therapy”. Trends in Molecular Medicine, 27(6), 524–537. https://doi.org/10.1016/J.MOLMED.2021.03.010. View PDF

Recent Review Articles

Lei, R., Akins, E. A., Wong, K. C. Y., Repina, N. A., Wolf, K. J., Dempsey, G. E., Schaffer, D. v., Stahl, A., & Kumar, S. (2021). “Multiwell Combinatorial Hydrogel Array for High-Throughput Analysis of Cell-ECM Interactions”. ACS Biomaterials Science and Engineering, 7(6), 2453–2465. https://doi.org/10.1021/ACSBIOMATERIALS.1C00065/ASSET/IMAGES/LARGE/AB1C00065_0006.JPEG. View PDF

Ramasubramanian, A., Muckom, R., Sugnaux, C., Fuentes, C., Ekerdt, B. L., Clark, D. S., Healy, K. E., & Schaffer, D. v. (2021). “High-Throughput Discovery of Targeted, Minimally Complex Peptide Surfaces for Human Pluripotent Stem Cell Culture”. ACS Biomaterials Science and Engineering, 7(4), 1344–1360. https://doi.org/10.1021/ACSBIOMATERIALS.0C01462/ASSET/IMAGES/LARGE/AB0C01462_0006.JPEG. View PDF

Cabral, K. A., Patterson, D. M., Scheideler, O. J., Cole, R., Abate, A. R., Schaffer, D. v., Sohn, L. L., & Gartner, Z. J. (2021). “Simple, affordable, and modular patterning of cells using DNA”. Journal of Visualized Experiments, 2021(168). https://doi.org/10.3791/61937. View PDF

Rosenbloom, A. B., Tarczynski, M., Lam, N., Kane, R. S., Bugaj, L. J., & Schaffer, D. v. (2020). “β-Catenin signaling dynamics regulate cell fate in differentiating neural stem cells”. Proceedings of the National Academy of Sciences of the United States of America, 117(46), 28828–28837. https://doi.org/10.1073/PNAS.2008509117/SUPPL_FILE/PNAS.2008509117.SAPP.PDF. View PDF

Mukherjee, A., Sudrik, C., Hu, Y., Arha, M., Stathos, M., Baek, J., Schaffer, D. v., & Kane, R. S. (2020). “CL6mN: Rationally Designed Optogenetic Photoswitches with Tunable Dissociation Dynamics”. ACS Synthetic Biology, 9(9), 2274–2281. https://doi.org/10.1021/ACSSYNBIO.0C00362/ASSET/IMAGES/LARGE/SB0C00362_0006.JPE. View PDF

Carneiro, A., Lee, H., Lin, L., van Haasteren, J., & Schaffer, D. v. (2020). “Novel Lung Tropic Adeno-Associated Virus Capsids for Therapeutic Gene Delivery”. Human Gene Therapy, 31(17–18), 996–1009. https://doi.org/10.1089/HUM.2020.169/ASSET/IMAGES/LARGE/HUM.2020.169_FIGURE3.JPEG. View PDF

Muckom, R. J., Sampayo, R. G., Johnson, H. J., Schaffer, D. v, Muckom, R. J., Sampayo, R. G., Schaffer, D. v, Johnson, H. J., & Schaffer Graduate Program in Bioengineering, D. v. (2020). “Advanced Materials to Enhance Central Nervous System Tissue Modeling and Cell Therapy”. Advanced Functional Materials, 30(48), 2002931. https://doi.org/10.1002/ADFM.202002931. View PDF

Muckom, R., Bao, X., Tran, E., Chen, E., Murugappan, A., Dordick, J. S., Clark, D. S., & Schaffer, D. v. (2020). “High-throughput 3D screening for differentiation of hPSC-derived cell therapy candidates”. Science Advances, 6(32). https://doi.org/10.1126/SCIADV.AAZ1457/SUPPL_FILE/AAZ1457_SM.PDF. View PDF

van Haasteren, J., Li, J., Scheideler, O. J., Murthy, N., & Schaffer, D. v. (2020). “The delivery challenge: fulfilling the promise of therapeutic genome editing”. Nature Biotechnology 2020 38:7, 38(7), 845–855. https://doi.org/10.1038/s41587-020-0565-5. View PDF

Repina, N. A., Johnson, H. J., McClave, T., Kane, R. S., & Schaffer, D. v. (2020). “Protocol to Fabricate Engineered Illumination Devices for Optogenetic Control of Cellular Signaling Dynamics”. STAR Protocols, 1(3), 100141. https://doi.org/10.1016/J.XPRO.2020.100141. View PDF

Repina, N. A., McClave, T., Johnson, H. J., Bao, X., Kane, R. S., & Schaffer, D. v. (2020). “Engineered Illumination Devices for Optogenetic Control of Cellular Signaling Dynamics”. Cell Reports, 31(10), 107737. https://doi.org/10.1016/J.CELREP.2020.107737. View PDF

Kulkarni, R. U., Gest, A. M. M., Lam, C. K., Raliski, B. K., James, F., Adil, M. M., Schaffer, D. v, Wang, Y., & Miller, E. W. (2020). Computationally Assisted Design of High Signal-to-Noise Photoinduced Electron Transfer-Based Voltage-Sensitive Dyes. https://doi.org/10.26434/CHEMRXIV.12401753.V1. View PDF

Byrne, L. C., Day, T. P., Visel, M., Strazzeri, J. A., Fortuny, C., Dalkara, D., Merigan, W. H., Schaffer, D. v., & Flannery, J. G. (2020). “In vivo–directed evolution of adeno-associated virus in the primate retina”. JCI Insight, 5(10). https://doi.org/10.1172/JCI.INSIGHT.135112. View PDF

Scheideler, O. J., Yang, C., Kozminsky, M., Mosher, K. I., Falcón-Banchs, R., Ciminelli, E. C., Bremer, A. W., Chern, S. A., Schaffer, D. v., & Sohn, L. L. (2020). “Recapitulating complex biological signaling environments using a multiplexed, DNA-patterning approach”. Science Advances, 6(12), 5696–5714. https://doi.org/10.1126/SCIADV.AAY5696/SUPPL_FILE/AAY5696_SM.PDF. View PDF

Mukherjee, A., Stathos, M. E., Varner, C., Arsiwala, A., Frey, S., Hu, Y., Smalley, D. M., Schaffer, D. v., & Kane, R. S. (2020). “One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway”. Chemical Communications, 56(25), 3685–3688. https://doi.org/10.1039/D0CC00920B. View PDF

Kang, P. H., Schaffer, D. v., & Kumar, S. (2020). “Angiomotin links ROCK and YAP signaling in mechanosensitive differentiation of neural stem cells”. Molecular Biology of the Cell, 31(5), 386–396. https://doi.org/10.1091/MBC.E19-11-0602/ASSET/IMAGES/LARGE/MBC-31-386-G006.JPEG. View PDF

Kim, S. H., Lee, S., Lee, H., Cho, M., Schaffer, D. v., & Jang, J. H. (2019). AAVR-Displaying Interfaces: Serotype-Independent Adeno-Associated Virus Capture and Local Delivery Systems. Molecular Therapy – Nucleic Acids, 18, 432–443. https://doi.org/10.1016/J.OMTN.2019.09.015. View PDF

Bao, X., Adil, M. M., Muckom, R., Zimmermann, J. A., Tran, A., Suhy, N., Xu, Y., Sampayo, R. G., Clark, D. S., & Schaffer, D. v. (2019). Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing. Stem Cells, 37(12), 1556–1566. https://doi.org/10.1002/STEM.3096. View PDF

Qiao EL, Kumar S, Schaffer DV. Mastering their own fates through the matrix. Nat Mater. 2019 Aug; 18(8):779-780. doi: 10.1038/s41563-019-0429-x. PubMed PMID: 31332317. View PDF

Recent Selected Publications

Qiao, E. L., Kumar, S., & Schaffer, D. v. (2019). Mastering their own fates through the matrix. Nature Materials 2019 18:8, 18(8), 779–780. https://doi.org/10.1038/s41563-019-0429-x. View PDF

Muckom R, McFarland S, Yang C, Perea B, Gentes M, Murugappan A, Tran E, Dordick JS, Clark DS, Schaffer DV. High-throughput combinatorial screening reveals interactions between signaling molecules that regulate adult neural stem cell fate. Biotechnol Bioeng. 2019 Jan; 116(1):193-205. doi: 10.1002/bit.26815. Epub 2018 Nov 6. PubMed PMID: 30102775; PubMed Central PMCID: PMC6289657. View PDF

Recent Selected Publications

Ekman, F. K., Ojala, D. S., Adil, M. M., Lopez, P. A., Schaffer, D. v., & Gaj, T. (2019). CRISPR-Cas9-Mediated Genome Editing Increases Lifespan and Improves Motor Deficits in a Huntington’s Disease Mouse Model. Molecular Therapy – Nucleic Acids, 17, 829–839. https://doi.org/10.1016/J.OMTN.2019.07.009. View PDF

Chen, S., Sun, S., Moonen, D., Lee, C., Lee, A. Y. F., Schaffer, D. v., & He, L. (2019). CRISPR-READI: Efficient Generation of Knockin Mice by CRISPR RNP Electroporation and AAV Donor Infection. Cell Reports, 27(13), 3780-3789.e4. https://doi.org/10.1016/J.CELREP.2019.05.103. View PDF

Nierode GJ, Gopal S, Kwon P, Clark DS, Schaffer DV, Dordick JS. High-throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture. Biotechnol Bioeng. 2019 Jan; 116(1):168-180. doi: 10.1002/bit.26839. Epub 2018 Oct 27. PubMed PMID: 30229860; PubMed Central PMCID: PMC6289706. View PDF

Recent Selected Publications

Zimmermann, J. A., & Schaffer, D. v. (2019). Engineering biomaterials to control the neural differentiation of stem cells. Brain Research Bulletin, 150, 50–60. https://doi.org/10.1016/J.BRAINRESBULL.2019.05.007. View PDF

Barnes, C., Scheideler, O., & Schaffer, D. (2019). Engineering the AAV capsid to evade immune responses. Current Opinion in Biotechnology, 60, 99–103. https://doi.org/10.1016/J.COPBIO.2019.01.002. View PDF

Sun S, Schaffer DV. Corrigendum to "Engineered viral vectors for functional interrogation, deconvolution, and manipulation of neural circuits" [Curr. Opin. Neurobiol. 50 (2018) 163-170]. Curr Opin Neurobiol. 2018 Dec; 53:220. doi: 10.1016/j.conb.2018.08.005. Epub 2018 Sep 5. PubMed PMID: 30196036. View PDF
Recent Selected Publications
Johnson, H. J., Chakraborty, S., Muckom, R. J., Balsara, N. P., & Schaffer, D. V. (2022). "A scalable and tunable thermoreversible polymer for 3D human pluripotent stem cell biomanufacturing". IScience, 25(10), 104971. https://doi.org/10.1016/J.ISCI.2022.104971. View PDF
Nierode, G. J., Gopal, S., Kwon, P., Clark, D. S., Schaffer, D. v., & Dordick, J. S. (2019). High-throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture. Biotechnology and Bioengineering, 116(1), 168–180. https://doi.org/10.1002/BIT.26839. View PDF
Adil MM, Schaffer DV. Cheaper and less variable expansion. Nat Biomed Eng. 2018 Mar; 2(3):144-145. doi: 10.1038/s41551-018-0209-y. PubMed PMID: 31015716. View PDF
Recent Selected Publications
Muckom, R., McFarland, S., Yang, C., Perea, B., Gentes, M., Murugappan, A., Tran, E., Dordick, J. S., Clark, D. S., & Schaffer, D. v. (2019). High-throughput combinatorial screening reveals interactions between signaling molecules that regulate adult neural stem cell fate. Biotechnology and Bioengineering, 116(1), 193–205. https://doi.org/10.1002/BIT.26815. View PDF
Adil MM, Schaffer DV. hPSC-derived Midbrain Dopaminergic Neurons Generated in a Scalable 3-D Biomaterial. Curr Protoc Stem Cell Biol. 2018 Feb 28; 44:2D.21.1-2D.21.17. doi: 10.1002/cpsc.47. PubMed PMID: 29512105. View PDF
Recent Selected Publications
Day TP, Byrne LC, Flannery JG, Schaffer DV. Screening for Neutralizing Antibodies Against Natural and Engineered AAV Capsids in Nonhuman Primate Retinas. Methods Mol Biol. 2018; 1715:239-249. doi: 10.1007/978-1-4939-7522-8_17. PubMed PMID: 29188518. View PDF
Recent Selected Publications
Halperin SO, Tou CJ, Wong EB, Modavi C, Schaffer DV, Dueber JE. CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window. Nature. 2018 Aug; 560(7717):248-252. doi: 10.1038/s41586-018-0384-8. Epub 2018 Aug 1. PubMed PMID: 30069054. View PDF
Recent Selected Publications
Dynamics of Mechanosensitive Neural Stem Cell Differentiation Corrigendum. View PDF
Fuentes, C. M., & Schaffer, D. V. (2018). "Adeno-associated virus-mediated delivery of CRISPR-Cas9 for genome editing in the central nervous system". Current Opinion in Biomedical Engineering, 7, 33–41. https://doi.org/10.1016/J.COBME.2018.08.003. View PDF
Sun, S., & Schaffer, D. v. (2018). "Engineered viral vectors for functional interrogation, deconvolution, and manipulation of neural circuits". Current Opinion in Neurobiology, 50, 163–170. https://doi.org/10.1016/J.CONB.2017.12.011. View PDF
Schaffer, D., & Shvartsman, S. Y. (2018). "Editorial overview: Tissue, cell and pathway engineering". Current Opinion in Biotechnology, 52, iv–v. https://doi.org/10.1016/J.COPBIO.2018.07.004. View PDF
Recent Review Articles
Ojala, D. S., Sun, S., Santiago-Ortiz, J. L., Shapiro, M. G., Romero, P. A., & Schaffer, D. v. (2018). "In Vivo Selection of a Computationally Designed SCHEMA AAV Library Yields a Novel Variant for Infection of Adult Neural Stem Cells in the SVZ". Molecular Therapy, 26(1), 304–319. https://doi.org/10.1016/J.YMTHE.2017.09.006. View PDF
Mukherjee, A., Dhar, N., Stathos, M., Schaffer, D. v., & Kane, R. S. (2018). "Understanding How Wnt Influences Destruction Complex Activity and β-Catenin Dynamics". IScience, 6, 13–21. https://doi.org/10.1016/J.ISCI.2018.07.007. View PDF
Mosher, K. I., & Schaffer, D. v. (2018b). "Proliferation versus Differentiation: Redefining Retinoic Acid’s Role". Stem Cell Reports, 10(6), 1673–1675. https://doi.org/10.1016/J.STEMCR.2018.05.011. View PDF
Mosher, K. I., & Schaffer, D. v. (2018a). "Influence of hippocampal niche signals on neural stem cell functions during aging". Cell and Tissue Research, 371(1), 115–124. https://doi.org/10.1007/S00441-017-2709-6/TABLES/1. View PDF
Hauser, M., Yan, R., Li, W., Repina, N. A., Schaffer, D. v., & Xu, K. (2018). "The Spectrin-Actin-Based Periodic Cytoskeleton as a Conserved Nanoscale Scaffold and Ruler of the Neural Stem Cell Lineage". Cell Reports, 24(6), 1512–1522. https://doi.org/10.1016/J.CELREP.2018.07.005. View PDF
Halperin, S. O., Tou, C. J., Wong, E. B., Modavi, C., Schaffer, D. v., & Dueber, J. E. (2018). "CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window". Nature 2018 560:7717, 560(7717), 248–252. https://doi.org/10.1038/s41586-018-0384-8. View PDF
Ekerdt, B. L., Fuentes, C. M., Lei, Y., Adil, M. M., Ramasubramanian, A., Segalman, R. A., & Schaffer, D. v. (2018). "Thermoreversible Hyaluronic Acid-PNIPAAm Hydrogel Systems for 3D Stem Cell Culture". Advanced Healthcare Materials, 7(12), 1800225. https://doi.org/10.1002/ADHM.201800225. View PDF
Dong, M., Spelke, D. P., Lee, Y. K., Chung, J. K., Yu, C. H., Schaffer, D. v., & Groves, J. T. (2018). "Spatiomechanical Modulation of EphB4-Ephrin-B2 Signaling in Neural Stem Cell Differentiation". Biophysical Journal, 115(5), 865–873. https://doi.org/10.1016/J.BPJ.2018.06.031. View PDF
Day, T. P., Byrne, L. C., Flannery, J. G., & Schaffer, D. v. (2018). "Screening for neutralizing antibodies against natural and engineered AAV capsids in nonhuman primate retinas". Methods in Molecular Biology, 1715, 239–249. https://doi.org/10.1007/978-1-4939-7522-8_17/FIGURES/1. View PDF
Chen, K. S., Xu, M., Zhang, Z., Chang, W. C., Gaj, T., Schaffer, D. v., & Dan, Y. (2018). "A Hypothalamic Switch for REM and Non-REM Sleep". Neuron, 97(5), 1168-1176.e4. https://doi.org/10.1016/J.NEURON.2018.02.005. View PDF
Adil, M. M., & Schaffer, D. v. (2018b). "Cheaper and less variable expansion". Nature Biomedical Engineering 2018 2:3, 2(3), 144–145. https://doi.org/10.1038/s41551-018-0209-y. View PDF
Adil, M. M., & Schaffer, D. v. (2018a). "hPSC-derived Midbrain Dopaminergic Neurons Generated in a Scalable 3-D Biomaterial". Current Protocols in Stem Cell Biology, 44(1), 2D.21.1-2D.21.17. https://doi.org/10.1002/CPSC.47. View PDF
Adil, M. M., Gaj, T., Rao, A. T., Kulkarni, R. U., Fuentes, C. M., Ramadoss, G. N., Ekman, F. K., Miller, E. W., & Schaffer, D. v. (2018). "hPSC-Derived Striatal Cells Generated Using a Scalable 3D Hydrogel Promote Recovery in a Huntington Disease Mouse Model". Stem Cell Reports, 10(5), 1481–1491. https://doi.org/10.1016/J.STEMCR.2018.03.007. View PDF
Adil, M. M., Rao, A. T., Ramadoss, G. N., Chernavsky, N. E., Kulkarni, R. U., Miller, E. W., Kumar, S., Schaffer, D. v, Adil, M. M., Ramadoss, G. N., Kumar, S., Schaffer, D. v, Rao, A. T., Miller, E. W., Chernavsky, N. E., & Kulkarni, R. U. (2018). "Dopaminergic Neurons Transplanted Using Cell-Instructive Biomaterials Alleviate Parkinsonism in Rodents". Advanced Functional Materials, 28(41), 1804144. https://doi.org/10.1002/ADFM.201804144. View PDF

Repina, N. A., Rosenbloom, A., Mukherjee, A., Schaffer, D. v., & Kane, R. S. (2017). “AT LIGHT SPEED: Advances in Optogenetic Systems for Regulating Cell Signaling and Behavior”. Annual Review of Chemical and Biomolecular Engineering, 8, 13. https://doi.org/10.1146/ANNUREV-CHEMBIOENG-060816-101254. View PDF

Gaj, T., Ojala, D. S., Ekman, F. K., Byrne, L. C., Limsirichai, P., & Schaffer, D. v. (2017). “In vivo genome editing improves motor function and extends survival in a mouse model of ALS”. Science Advances, 3(12). https://doi.org/10.1126/SCIADV.AAR3952/SUPPL_FILE/AAR3952_SM.PDF. View PDF

Kang, P. H., Kumar, S., & Schaffer, D. v. (2017a). “Making way for neural stemness”. Nature Materials 2017 16:12, 16(12), 1174–1176. https://doi.org/10.1038/nmat5043. View PDF

Mukherjee, A., Repina, N. A., Schaffer, D. v., & Kane, R. S. (2017). “Optogenetic tools for cell biological applications”. Journal of Thoracic Disease, 9(12), 4867. https://doi.org/10.21037/JTD.2017.11.73

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Kang, P. H., Kumar, S., & Schaffer, D. v. (2017b). “Novel biomaterials to study neural stem cell mechanobiology and improve cell-replacement therapies”. Current Opinion in Biomedical Engineering, 4, 13–20. https://doi.org/10.1016/J.COBME.2017.09.005

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Wooley, D. P., Sharma, P., Weinstein, J. R., Kotha Lakshmi Narayan, P., Schaffer, D. v., & Excoffon, K. J. D. A. (2017). “A directed evolution approach to select for novel Adeno-associated virus capsids on an HIV-1 producer T cell line”. Journal of Virological Methods, 250, 47–54. https://doi.org/10.1016/J.JVIROMET.2017.09.008

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Sinn, P. L., Hwang, B. Y., Li, N., Ortiz, J. L. S., Shirazi, E., Parekh, K. R., Cooney, A. L., Schaffer, D. v., & McCray, P. B. (2017). “Novel GP64 envelope variants for improved delivery to human airway epithelial cells”. Gene Therapy 2017 24:10, 24(10), 674–679. https://doi.org/10.1038/gt.2017.78

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Epstein, B. E., & Schaffer, D. v. (2017). “Combining engineered nucleases with adeno-associated viral vectors for therapeutic gene editing”. Advances in Experimental Medicine and Biology, 1016, 29–42. https://doi.org/10.1007/978-3-319-63904-8_2/TABLES/1

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Bergen, J., & Schaffer, D. (2017). “4.33 Engineering Viruses For Gene Therapy”. Comprehensive Biomaterials II, 600–616. https://doi.org/10.1016/B978-0-08-100691-7.00167-1. View PDF

Rodrigues, G. M. C., Gaj, T., Adil, M. M., Wahba, J., Rao, A. T., Lorbeer, F. K., Kulkarni, R. U., Diogo, M. M., Cabral, J. M. S., Miller, E. W., Hockemeyer, D., & Schaffer, D. v. (2017). “Defined and Scalable Differentiation of Human Oligodendrocyte Precursors from Pluripotent Stem Cells in a 3D Culture System”. Stem Cell Reports, 8(6), 1770–1783. https://doi.org/10.1016/J.STEMCR.2017.04.027. View PDF

Sanghvi, J. C., Mai, D., Arkin, A. P., & Schaffer, D. v. (2017). A comprehensive stochastic computational model of HIV infection from DNA integration to viral burst. https://doi.org/10.48550/arxiv.1711.11270. View PDF

Schaffer, D., & Willerth, S. (2017). “5.8 Scaffold Materials for Human Embryonic Stem Cell Culture and Differentiation”. Comprehensive Biomaterials II, 129–153. https://doi.org/10.1016/B978-0-12-803581-8.10111-0.  View PDF

Adil, M. M., Vazin, T., Ananthanarayanan, B., Rodrigues, G. M. C., Rao, A. T., Kulkarni, R. U., Miller, E. W., Kumar, S., & Schaffer, D. v. (2017). “Engineered hydrogels increase the post-transplantation survival of encapsulated hESC-derived midbrain dopaminergic neurons”. Biomaterials, 136, 1–11. https://doi.org/10.1016/J.BIOMATERIALS.2017.05.008.  View PDF

Gaj, T., Staahl, B. T., Rodrigues, G. M. C., Limsirichai, P., Ekman, F. K., Doudna, J. A., & Schaffer, D. v. (2017). “Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery”. Nucleic Acids Research, 45(11), e98–e98. https://doi.org/10.1093/NAR/GKX154. View PDF

Adil, M. M., Rodrigues, G. M. C., Kulkarni, R. U., Rao, A. T., Chernavsky, N. E., Miller, E. W., & Schaffer, D. v. (2017). “Efficient generation of hPSC-derived midbrain dopaminergic neurons in a fully defined, scalable, 3D biomaterial platform”. Scientific Reports 2017 7:1, 7(1), 1–11. https://doi.org/10.1038/srep40573. View PDF

Limsirichai, P. T. Gaj, and D.V. Schaffer (2016) "CRISPR-Mediated Activation of Latent HIV-1 Expression." (PMID 26607397). View PDF
Maroof M Adi; and David V Schaffer "Expansion of human pluripotent stem cells." View PDF
A Rationally Designed, General Strategy for Membrane Orientation of Photoinduced Electron Transfer-Based Voltage-Sensitive Dyes. View PDF
Nierode, G.J., B.C. Perea, S.K. McFarland, J.F. Pascoal, D.S. Clark, D.V. Schaffer, and J.S. Dordick (2016) "High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform." . View PDF
Gaj., T., B.E. Epstein, and D.V. Schaffer (2016), "Genome Engineering Using Adeno-Associated Virus: Basic and Clinical Research Applications." The American Society of Gene & Cell Therapy,1-7(PMID 26373345). View PDF
Recent Review Articles
Chen, S. Y.S. Na, M.E. Todhunger, S. Hsiao, A.W. Bremer, O.J. Scheideler, P.R. Bomdica, M.M. Haharbiz, Z.J. Gartner, D.V. Schaffer (2016) "Interrogating Stem Cell Fate Decisions with High-throughput Arrays of Cellular Communities." View PDF
Recent Selected Publications
Tervo, D.G.R., B-Y. Hwang, S. Viswanathan, T. Gaj, M. Lavzin, K.D. Ritola, S. Lindo, S. Michael, E.P. Kuleshova, D. Ojala, C-C. Huang, C.R. Gerfen, J. Schiller, J.T. Dudman, A.W. Hantman, L.L. Looger, D.V. Schaffer (co-corresponding author), and A.Y. Karpova (2016) "A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons." , 92:372-382 (PMID 27720486). View PDF
Recent Selected Publications
Dynamics of Mechanosensitive Neural Stem Cell Differentiation. View PDF
Luque, T., M.S. Kang, D.V. Schaffer, and S. Kumar (2016) "Microelastic Mapping of the Rat Dentate Gyrus." 3:150702 (PMID: 27152213). View PDF
Zheng. B., M.P. von See, E. Yu, B. Gunel, K. Lu, T. Vazin, D.V. Schaffer, P.W. Goodwill, and S.M. Conolly (2016) "Quantitative Magnetic Particle Imaging Monitors the Transplantation, Biodistribution, and Clearance of Stem Cells in Vivo." 6:291-301 (PMID: 26909106). View PDF
Rammensee, S., M.S. Kang, K. Georgiou, S. Kumar, and D.V. Schaffer (2016) "Dynamics of Mechanosensitive Neural Stem Cell Differentiation." , 2016 Aug 30. doi: 10.1002/stem.2489 (PMID: 27573749). View PDF
Tsukamoto, A. S.E. Abbot, L.C. Kadyk, N.D. DeWitt, D.V. Schaffer, J.A. Wertheim, K.J. Whittlesey, M.J. Werner (2016) Regeneration to Transform Medicine." (PMID 26607174).
Altiok, E.I., J.L. Santiago-Ortiz, F.L. Svedlund, A. Zbinden, A.K. Jha, D. Bhatnagar, P. Loskill, W.M. Jackson, D.V. Schaffer, and K.E. Healy (2016) "Multivalent Hyaluronic Acid Bioconjugates Improve sFlt-1 activity in Vitro." 93:95-105 (PMID: 27086270). View PDF
Zheng. B., M.P. von See, E. Yu, B. Gunel, K. Lu, T. Vazin, D.V. Schaffer, P.W. Goodwill, and S.M. Conolly (2016) "Quantitative Magnetic Particle Imaging Monitors the Transplantation, Biodistribution, and Clearance of Stem Cells in Vivo." , 6:291-301 (PMID 26909106). View PDF
Santiago-Ortiz, J.L. and D.V. Schaffer (2016) "Adeno-associated Virus (AAV) Vectors in Cancer Gene Therapy" Jan 12. pii: S0168-3659(16)30001-3 (PMID: 26796040). View PDF
Steines, B., D.D. Dickey, J. Bergen, K.J.D.A Excoffon, J.R. Weinstein, X. Li, Z. Yan, M.H. Abou Alaiwa, V.S. Shah, D.C. Bouzek, L.S. Powers, N.D. Gansemer, L.S. Ostedgaard, J.F. Engelhardt, D.A. Stoltz, M.J. Welsh, P.L. Sinn, D.V. Schaffer (co-corresponding author), and J. Zabner (2016) "CFTR Gene-transfer with AAV Improves Early Cystic Fibrosis Pig Phenotypes." , 1:e88728 (PMID 27699238). View PDF
Yao, K., S. Qiu, L. Tian, W.D. Snider, J.G. Flannery, D.V. Schaffer, and B. Chen (2016) "Wnt Regulates Proliferation and Neurogenic Potential of Müller Glial Cells via a Lin28/let-7 miRNA-Dependent Pathway in Adult Mammalian Retinas." , 17:165-178 (PMID 27681429). View PDF
Deng Y, Wang H, Hamamoto R, Schaffer D, Duan S (2015) “Functional genomics, genetics, and bioinformatics.” Biomed Res Int, 2015:184824 (PMID 25977917). View PDF
Ojala, D., D.P. Amara, and D.V. Schaffer (2015) "AAV Vectors and Neurological Gene Therapy." Neuroscientist, 21(1): 84-98. View PDF
Kotterman, M.A., T. Vazin, and D.V. Schaffer (2015) "Adeno-Associated Viral Variant Enhances Selective Gene Delivery to Neural Stem Cells in Vivo." Development. View PDF
Byrne, L.C., Y.J. Lin, T. Lee, D.V. Schaffer, and J.G. Flannery (2015) "The Expression Pattern of Systemically Injected AAV9 in the Developing Mouse Retina is Determined by Age." Molecular Therapy, 23(2): 290-6 (PMID 25224467). View PDF
Bugaj, L.J., D. P. Spelke, C.K. Mesuda, M. Varedi, R.S. Kane, and D.V. Schaffer (2015)"Regulation of Endogenous Transmembrane Receptors Through Optogenetic Cry2 Clustering." Nature Communications. View PDF
Recent Selected Publications
Kotterman, M.A., L. Yin, J.M. Strazzeri, J.G. Flannery, W.M. Merigan, and D.V. Schaffer (2015) "Antibody Neutralization Poses a Barrier to Intravitreal Adeno-Associated Viral Vector Gene Delivery to Non-Human Primates." Gene Therapy, 22(2):116-26 (PMID 25503696). View PDF
Fritz, A.L., M.M. Adil, S.R. Mao, D.V. Schaffer (2015) "cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming." , 23:952-963 (PMID 25666918). View PDF
Yousef, H., A. Morgenthaler, C. Schlesinger, L. Bugaj, I. Conboy, and D.V. Schaffer (2015) "Age-Associated Increase in BMP Signaling inhibits Hippocampal Neurogenesis." Stem Cells (in press). View PDF
Santiago-Ortiz, J., D.S. Ojala, O. Westesson, J.R. Weinstein, S.Y. Wong, A. Steinsapir, S. Kumar, I. Holmes, and D.V. Schaffer (2015) "AAV Ancestral Reconstruction Library Enables Selection of Broadly Infectious Viral Variants." , 1-13 (PMID 26186661). View PDF
Han, B.W., H. Layman, N.A. Rode, A. Conway, D.V. Schaffer, N.J. Boudreau, W.M. Jackson, and K.E. Healy (2015) "Multivalent Conjugates of Sonic Hedgehog Accelerate Diabetic Wound Healing." , 21:2366-2378 (PMID 26154888). View PDF
Schaffer, D.V. (corresponding author), M.A. Kotterman, and T.W. Chalberg (2015) "Viral Vectors for Gene Therapy: Translational and Clinical Outlook." Annual Review of Biomedical Engineering View PDF
Dey, S.S., J.E. Foley, P. Limsirichai, D.V. Schaffer (co-corresponding author), and A.P. Arkin (2015) "Orthogonal Control of Expression Mean and Variance by Epigenetic Features at Different Genomic Loci" Molecular Systems Biology. View PDF
Yousef, H., M.J. Conboy, A. Morgenthaler, C. Schlesinger, L. Bugaj, P. Paliwal, C. Greer, I.M. Conboy, and D.V. Schaffer (2015) "Systemic Attenuation of the TGF-β Pathway by a Single Drug Simultaneously Rejuvenates Hippocampal Neurogenesis and Myogenesis in the Same Old Mammal." , 6:11959-11978 (PMID 26003168). View PDF
Jha, A.K., K.M. Tharp, J. Ye, J.L. Santiago-Ortiz, W.M. Jackson, A. Stahl, D.V. Schaffer, Y. Yeghiazarians, and K.E. Healy (2015)"Enhanced Survival and Engraftment of Transplanted Stem Cells Using Growth Factor Sequestering Hydrogels." Biomaterials, 47:1-12 (PMID 25682155). View PDF
Fritz, A.L., S.R. Mao, M.G. West, and D.V. Schaffer (2015) "A Medium-Throughput Analysis of Signaling Pathways Involved in Early Stages of Stem Cell Reprogramming." Biotechnology & Bioengineering, 112 (1): 209-19 (PMID 25065366). View PDF
Zheng, V., T. Vazin, P.W. Goodwill, A. Conway, A. Verma, E. Ulku Saritas, D.V. Schaffer, and S.M. Conolly (2015) "Magnetic Particle Imaging Tracks the Long-term Fate of in Vivo Neural Cell Implants with High Image Contrast." , 5:14055 (PMID 26358296). View PDF
Kotterman, M.A. and D.V. Schaffer (2014) "Engineering Adeno-Associated Viruses for Clinical Gene Therapy." Nature Reviews Genetics, 15:445-451 (PMID 24840552). View PDF
Recent Review Articles Recent Selected Publications
Shapiro, M.G., R.M. Ramirez, L.J. Sperling, G. Sun, A. Pines, D.V. Schaffer, and V.S. Bajaj (2014) "Genetically Encoded Reporters for Hyperpolarized Xenon." , 6(7):629-34 (PMID: 24950334). View PDF
Recent Selected Publications
Vacca, O., M. Darche, D.V. Schaffer, J.G. Flannery, J.A. Sahel, A. Rendon, and D. Dalkara (2014) "AAV-mediated Gene Delivery in Dp71-null Mouse Model with Compromised Barriers." , 62:468-476 (PMID 24382652). View PDF
Hughes, A.J., D.P. Spelke, Z. Xu, C-C. Kang, D.V. Schaffer (co-corresponding author), and A.E. Herr (2014) "Single-cell Western Blotting." , 11:749-755 (PMID 24880876). View PDF
Recent Selected Publications
Ojala, D., D.P. Amara, and D.V. Schaffer (2014) "AAV Vectors and Neurological Gene Therapy." (in press). View PDF
Shapiro, M., P. Goodwill, A. Neogy, M. Yin, F. Foster, D.V. Schaffer, and S. Conolly (2014) "Biogenic Gas Nanostructures as Ultrasonic Molecular Reporters." , 9:311-316 (PMID 24633522). View PDF
Recent Selected Publications
Byrne, L.C., B.E. Öztürk, T. Lee, C. Fortuny, M. Visel, D. Dalkara, D.V. Schaffer, and J.G. Flannery (2014) "Retinoschisin Gene Therapy in Photoreceptors Provides Long-term Rescue in the Rs1h-/- Mouse." , 21(6): 585-92 (PMID: 24694538). View PDF
Leo, Y., D. Jeong, J, Xiao, and D.V. Schaffer (2014) "Developing Defined and Scalable 3D Culture Systems for Culturing Human Pluripotent Stem Cells at High Densities." , Volume 7, Issue 2, pp 172-183 View PDF
Day, T.P., L.C. Byrne, D.V. Schaffer, and J.G. Flannery (2014) "Advances in AAV Vector Development for Gene Therapy in the Retina." , 801:687-693 (PMID 24664759). View PDF
Conway, A. and D.V. Schaffer (2014) "Biomaterial Microenvironments to Support the Generation of New Neurons in the Adult Brain." , 32:1220-1229 (PMID 24449485). View PDF
Cao, J., M. Ahra, C. Sudrik, D.V. Schaffer, and R.S. Kane (2014) "Bidirectional Regulation of mRNA Translation in Mammalian Cells by Using PUF Domains." , 5; 53 (19):4900-4 (PMID: 24677733). View PDF
Loring, J.F., T.C. McDevitt, S.P. Palecek, D.V. Schaffer, P.W. Zandstra, and R.M. Nerem (2014) "A Global Assessment of Stem Cell Engineering." , 20(19-20):2575-89 (PMID 24428577). View PDF
Schaffer, D.V. (2014) "AAV Shuffles to the Liver." , 14006. View PDF
Bartel, M.A. and D.V. Schaffer (2014) "Enhanced Gene Targeting of Adult and Pluripotent Stem Cells Using Evolved Adeno-Associated Virus." , 1114: 169-179 (PMID 24557903). View PDF
Yin, L., B. Masella, D. Dalkara, J. Zhang, J.G. Flannery, D.V. Schaffer, D.R. Williams, and W.H. Merigan (2014) "Imaging Light Responses of Foveal Ganglion Cells in the Living Macaque Eye." , 7; 34 (19) (PMID: 24806684). View PDF
Conway A., D.P. Spelke, and D.V. Schaffer (2014) "Conjugation of Proteins to Polymer Chains to Create Multivalent Molecules." , 1202:95-102 (PMID 24014311). View PDF
Vazin, T., R.S. Ashton, A. Conway, N. Rode, S.M. Lee, V. Bravo, K.E. Healy, R.S. Kane, and D.V. Schaffer (2014) "The Effect of Multivalent Sonic Hedgehog on Differentiation of Human Embryonic Stem Cells into Dopaminergic and GABAergic Neurons." , 35:941-948 (PMID 24172856). View PDF
Lei, Y. and D.V. Schaffer (2013) "A Fully Defined and Scalable 3D Culture System for Human Pluripotent Stem Cell Expansion and Differentiation." , 110:E5039-5048 (PMID 24248365). View PDF
Recent Selected Publications
Yousef, H., M.J. Conboy, J. Li, M. Zeiderman, T. Vazin, C. Schlesinger, D.V. Schaffer, and I.M. Conboy (2013) "hESC-Secreted Proteins can be Enriched for Multiple Regenerative Therapies by Heparin-Binding." , 5:357-372 (PMID 23793469). View PDF
Miller-Jensen, K., R. Skupsky, P.S. Shah, A.P. Arkin, and D.V. Schaffer (2013) "Genetic Selection for Context-Dependent Stochastic Phenotypes: Sp1 and TATA Mutations Increase Phenotypic Noise in HIV-1 Gene Expression." , 9:e1003135 (PMID 23874178). View PDF
Pernet, V., S. Joly, D. Dalkara, N. Jordi, O. Schwarz, F. Christ, D.V. Schaffer, J.G. Flannery, and M.E. Schwab (2013) "Long-distance Axonal Regeneration Induced by CNTF Gene Transfer is Impaired by Axonal Misguidance in the Injured Adult Optic Nerve." , 51:202-13 (PMID 23194670). View PDF
Keung, A.J., M. Dong, D.V. Schaffer (co-corresponding author), and S. Kumar (2013) "Pan-neuronal Maturation But Not Neuronal Subtype Differentiation of Adult Neural Stem Cells is Mechanosensitive." , 3:1817 (PMID 23660869). View PDF
Sudrik C., M. Arha, J. Cao, D.V. Schaffer (co-corresponding author), and R.S. Kane (2013) "Translational Repression Using BIV Tat peptide-TAR RNA Interaction in Mammalian Cells." , 49(67):7457-7459 (PMID 23852014). View PDF
Vazin, T., K.A. Ball, H. Lu, H. Park, Y. Ataeijannati, T. Head-Gordon, M-M Poo, and D. V. Schaffer (2013) "Efficient Derivation of Cortical Glutamatergic Neurons from Human Pluripotent Stem Cells: A Model System to Study Neurotoxicity in Alzheimer’s Disease." , 62C:62-72 (PMID 24055772). View PDF
Downing, T.L., J. Soto, C. Morez, T. Houssin, A. Fritz, F. Yuan, J. Chu, S. Patel, D.V. Schaffer, and S. Li (2013) "Biophysical Regulation of Epigenetic State and Cell Reprogramming." , 12:1154-1162 (PMID 24141451). View PDF
Recent Selected Publications
Dalkara, D., L.C. Byrne, R.R. Klimczak, M. Visel, L. Yin, W.H. Merigan, J.G. Flannery, and D.V. Schaffer (2013) "In Vivo–Directed Evolution of a New Adeno-Associated Virus for Therapeutic Outer-Retinal Gene Delivery from the Vitreous." , 5:189ra76 (PMID 23761039). View PDF
Recent Selected Publications
Perry, S.J., S.G. Neumann, T. Neumann, K. Cheng, J. Ni, J.R. Weinstein, D.V. Schaffer, and M. Tirrell "Challenges in Nucleic Acid-Lipid Films for Transfection" (in press).
Hwang, B-Y. and D.V. Schaffer (2013) "Engineering a Serum-resistant and Thermostable Vesicular Stomatitis Virus G Glycoprotein for Pseudotyping Retroviral and Lentiviral Vectors." , 20:807-815 (PMID 23364315). View PDF
Byrne, L.C., F. Khalid, T. Lee, E.A. Zin, K.P. Greenberg, M. Visel, D.V. Schaffer, and J.G. Flannery (2013) "AAV-mediated, Optogenetic Ablation of Müller Glia Leads to Structural and Functional Changes in the Mouse Retina." , 8:e76075 (PMID 24086689). View PDF
Conway, A., T. Vazin, D.P. Spelke, N.A. Rode, K.E. Healy, R.S. Kane, and D.V. Schaffer (2013) "Multivalent Ligands to Control Stem Cell Behaviour in Vitro and in Vivo." , 8:831-838 (PMID 24141540). View PDF
Recent Selected Publications
Bugaj, L.J, A.T Choksi, C.K. Mesuda, R.S. Kane, and D.V. Schaffer (2013) "Optogenetic Protein Clustering and Signaling Activation in Mammalian Cells." , 10:249-252. View PDF
Recent Selected Publications
Ekerdt, B.L., R.A. Segalman, and D.V. Schaffer (2013) "Spatial Organization of Cell-Adhesive Ligands for Advanced Cell Culture." , 8:1411-1423 (PMID 24318636).
Cao, J., M. Arha, C. Sudrik, L.J. Bugaj, D.V. Schaffer (co-corresponding author), and R.S. Kane (2013) "Light-inducible Activation of Target mRNA Translation in Mammalian Cells" , 49:8338-8340 (PMID 23925486). View PDF
Chen, S. and D.V. Schaffer (2013) "Newborn Neuroblasts Feel the Field in the Adult Brain." , 14:105-106 (PMID 23328741). View PDF
Bartel, M.A., J.R. Weinstein, and D.V. Schaffer (2012) "Directed Evolution of Novel Adeno-Associated Viruses for Therapeutic Gene Delivery." Gene Therapy (2012) 19, 19:694-700 (PMID 22402323). View PDF
Recent Review Articles
Conway, A. and D.V. Schaffer (2012) "Biophysical Regulation of Stem Cell Behavior in the Niche." , 3:50 (PMID 23241436). View PDF
Bugaj, L.J. and D.V. Schaffer (2012) "Bringing Next-Generation Therapeutics to the Clinic Through Synthetic Biology." , 16(3-4):355-361 (PMID: 22609142). View PDF
Ana, H.J, P. Gip, J. Kimc, S. Wua, C.T. McVaugh, D.V. Schaffer, C.R. Bertozzi, and C.B. Lebirlla (2012) "N-Glycan Profiling of Human Embryonic Stem Cell Surfaces Reveals an Unusual Abundance of High-Mannose Glycans." , 11:M111.010660 (PMID 22147732). View PDF
Shah, P.S., N.P. Phan, and D.V. Schaffer (2012) "HIV Develops Indirect Cross-resistance to Combinatorial RNAi Targeting Two Distinct and Spatially Distant Sites." , 20:840-848 (PMID 22294151). View PDF
Miller-Jensen, K., S.S. Dey, N. Pham, J.E. Foley, A.P. Arkin, and D.V. Schaffer (2012) "Chromatin Accessibility at the HIV LTR Promoter Sets a Threshold for NF-κB Mediated Viral Gene Expression." , 4:661-671 (PMID 22555315). View PDF
Dalkara, D., L.C. Byrne, T. Lee, N.V. Hoffmann, D.V. Schaffer, and J.G. Flannery (2012) Enhanced Gene Delivery to the Neonatal Retina Through Systemic Administration of Tyrosine-Mutated AAV9." , 19:176-181 (PMID: 22011645). View PDF
Asuri, P., M.A. Bartel, T. Vazin, J-H. Jang, T.B. Wong, and D.V. Schaffer (2012) "Directed Evolution of Adeno-Associated Virus for Enhanced Gene Delivery and Gene Targeting in Human Pluripotent Stem Cells." , 20(2):329-38 (PMID 22108859). View PDF
Ashton, R.S., A. Conway, C. Pangarkar, J. Bergen, K-I. Lim, P. Shah, M. Bissell, and D.V. Schaffer (2012) "Astrocytes Regulate Adult Hippocampal Neurogenesis Through Ephrin-B Signaling." , 15:1399-1406 (PMID: 22983209). View PDF
Recent Selected Publications
Jgamadze, J., J. Bergen, D. Stone, J-H. Jang, D.V. Schaffer, E.Y. Isacoff, and S. Pautot (2012) "Colloids as Mobile Substrates for the Implantation and Integration of Differentiated Neurons into the Mammalian Brain." , 7:e30293 (PMID 22295079). View PDF
Asokan, A., D.V. Schaffer, and R.J. Samulski (2012) "The AAV Vector Toolkit: Poised at the Clinical Crossroads." The American Society of Gene & Cell Therapy, 20:699-708 (PMID 22273577). View PDF
Recent Review Articles
Pollock, J., R. Aston, N. Rode, D.V. Schaffer, K.E. Healy (2012) "Molecular Characterization of Multivalent Bioconjugates by Size-Exclusion Chromatography (SEC) with Multi-Angle Laser Light Scattering (MALS)." , 19; 23(9):1794-801 (PMID: 22794081). View PDF
Keung, A.J., P. Asuri, S. Kumar, and D.V. Schaffer (2012) "Soft Microenvironments Promote the Early Neurogenic Differentiation but not Self-renewal of Human Pluripotent Stem Cells." , 4(9):1049-1058 (PMID: 22854634). View PDF
Goodwill, P.W., E.U. Saritas, L.R. Croft, T.N. Kim, K.M. Krishnan, D.V. Schaffer, and S.M. Conolly (2012) "X-space MPI: Magnetic Nanoparticles for Safe Medical Imaging." , 24:3870-3877 (PMID 22988557). View PDF
Dey, S.S., Y. Xue, M.P. Joachimiak, G.D. Friedland, J.C. Burnett, Q. Zhou, A.P. Arkin, and D.V. Schaffer (2012) "Mutual Information Analysis Reveals Coevolving Residues in Tat that Compensate for Two Distinct Functions in HIV-1 Gene Expression." , 287:7945-7955 (PMID 22253435). View PDF
Perneta, V., S. Joly, D. Dalkara, O. Schwarz, Franziska Christ, D. Schaffer, J.G. Flannery, and M.E. Schwab "Neuronal NOGO-A Up-Regulation Does Not Contribute to ER Stress-Associated Apoptosis but Participates in the Regenerative Response in the Axotomized Adult Retina." , 19:1096-1108 (PMID 22193546). View PDF
Bartel, M.A., D.V. Schaffer (co-corresponding author), and H. Buening (2011). "Enhancing the Clinical Potential of AAV Vectors by Capsid Engineering to Evade Pre-Existing Immunity." , 2:204 (PMID 22065962). View PDF
Dickey, D., K. Excoffon, J. Koerber, J. Bergen, B. Steines, J. Klesney-Tait, D.V. Schaffer, and J. Zabner (2011) "Enhanced Sialic Acid-dependent Endocytosis Explains the Increased Airway Epithelia Infection Efficiency of a Novel Adeno-associated Virus." , 85:9023-9030 (PMID 21697483). View PDF
Peltier, J., A. Conway, A. Keung, and D.V. Schaffer (2011) "Akt Increases Sox2 Expression in Adult Hippocampal Neural Progenitor Cells but Increased Sox2 does not Promote Proliferation." , 20:1153-1161 (PMID 21028992).
Dalkara, D., K.D. Kolstad, K.I. Guerin, N.V. Hoffmann, M. Visel, R.R. Klimczak, D.V. Schaffer, and J.G. Flannery (2011) "AAV-mediated GDNF Secretion from Retinal Glia Slows Down Retinal Degeneration in a Rat Model of Retinitis Pigmentosa." , 19:1602-1608 (PMID 21522134). View PDF
Dickinson, B.C., J. Peltier, D. Stone, D.V. Schaffer (co-corresponding author), and C.J Chang (2011) "Nox2 Redox Signaling Maintains Essential Cell Populations in the Brain." , 7:106-112 (PMID 21186346). View PDF
Recent Selected Publications
Jang, J-H., D.V. Schaffer, and L. Shea (2011) "Engineering Biomaterial Systems to Enhance Viral Vector Gene Delivery." , 19:1407-1415 (PMID 21629221). View PDF
Arkin, A.P. and D.V. Schaffer (2011) "Network News: Innovations in 21st Century Systems Biology." , 144:844-849 (PMID 21414475). View PDF
Jang, J-H., J.T. Koerber, J-S. Kim, P. Asurim, T. Vazin, M. Bartel, A. Keung, I. Kwon, K.I. Park, and D.V. Schaffer (2011) "An Evolved Adeno-Associated Viral Variant Enhances Gene Delivery and Gene Targeting in Neural Stem Cells." , 19:667-675 (PMID 21224831).http://www.cchem.berkeley.edu/schaffer/2011 Publications/Jang J.pdf View PDF
Keung, A.J., E.M. de Juan-Pardo, D.V. Schaffer (co-corresponding author), and S. Kumar (2011) "Rho GTPases Mediate the Mechanosensitive Lineage Commitment of Neural Stem Cells." , 29: 1886-1897 (PMID 21956892). View PDF
Chen, S., N. Patel, D.V. Schaffer, and M.M. Maharbiz (2011) "Trap and Corral: A Two-step Approach for Constructing and Constraining Dynamic Cell Contact Events in Differentiating Progenitor Cell Populations." , 5:054027. View PDF
Miller-Jensen, K., S.S. Dey, D.V. Schaffer, and A.P. Arkin (2011) "Varying Virulence: Epigenetic Control of Expression Noise and Disease Processes." , 29:517-525 (PMID 21700350). View PDF
Ashton, R.A., A.J. Keung, J.L. Peltier, and D.V. Schaffer (2011) "Progress and Prospects for Stem Cell Engineering." , 2:479-509. View PDF
Yin, Y., K. Greenberg, J.J. Hunter, D. Dalkara, K.D. Kolstad, B.D. Masella, R. Wolfe, M. Visel, D. Stone, R.T. Libby, D. DiLoreto, Jr., D. Schaffer, J. Flannery, D.R. Williams, W.H. Merigan (2011) "Intravitreal Injection of AAV2 Transduces Macaque Inner Retina." , 52:2775-2783 (PMID 21310920). View PDF
Shah, P.S. and D.V. Schaffer (2011) "Antiviral RNAi: Translating Science Toward Therapeutic Success." , 28:2966-2982 (PMID 21826573). View PDF
Hester, M.E., M.J. Murtha, S. Song, M. Rao, C.J. Miranda, K. Meyer, J. Tian, G. Boulting, D.V. Schaffer, M.X. Zhu, S.L. Pfaff, F.H. Gage, and B.K. Kaspar (2011) "Rapid and Efficient Generation of Functional Motor Neurons from Human Pluripotent Stem Cells Using Gene Delivered Transcription Factor Codes." , 19:1905-1912(PMID 21772256). View PDF
Schaffer, D.V. (2010) "Microarraying for Mechanosensitivity." , 7:273-4 (PMID 20804961). View PDF
Peltier, J. and D.V. Schaffer (2010) "Systems Biology Approaches to Understanding Stem Cell Fate Choice.", 4:1-11 (PMID 20001088). View PDF
Kolstad, K.D., D. Dalkara, K. Guerin, M. Visel, N. Hoffmann, D.V. Schaffer, and J.G. Flannery (2010) "Changes to AAV Mediated Gene Delivery in Retinal Degeneration." , 5:571-8 (PMID 20021232).
Dickinson, B.C., J. Peltier, D. Stone, D.V. Schaffer (co-corresponding author), and C.J Chang "Nox2 Redox Signaling Maintains Essential Cell Populations in the Brain." , 7:106-112(PMID 21186346). View PDF
Keung, A.J., S. Kumar, and D.V. Schaffer (2010) "Presentation Counts: Microenvironmental Regulation of Stem Cells by Biophysical and Material Cues." Annu. Rev. Cell Dev. Biol. 2010. 26:533-556 (PMID 20590452). View PDF
Recent Review Articles
Peltier, J. and D.V. Schaffer (2010) "Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors." , 621:103-116 (PMID 20405362). View PDF
Vazin, T. and D.V. Schaffer (2010) "Engineering Strategies to Emulate the Stem Cell Niche Trends in Biotechnology." Trends in Biotechnology Vol.28 No.3, 28:117-124 (PMID 20042248). Selected as the Cover Article. View PDF
Recent Review Articles
Ananthanarayanan, B., L. Little, D.V. Schaffer, K.E. Healy, and M. Tirrell (2010) "Neural Stem Cell Adhesion and Proliferation on Phospholipid Bilayers Functionalized with RGD Peptides." , 31:8706-15 (PMID 20728935). View PDF
Jang, J-H., J.T. Koerber, K. Gujraty, S.R. Bethi, R.S. Kane, and D.V. Schaffer "Surface Immobilization of Hexa-histidine-tagged Adeno-associated Viral Vectors for Localized Gene Delivery." , 17:1384-1389 (PMID 20508598). View PDF
Peltier, J., A. Conway, A. Keung, and D.V. Schaffer (2010) "Akt Increases Sox2 Expression in Adult Hippocampal Neural Progenitor Cells but Increased Sox2 does not Promote Proliferation." (PMID 21028992).
Little, L., K. Dane, P.S. Daugherty, K.E. Healy, and D.V Schaffer (2011) "Exploiting Bacterial Peptide Display Technology to Engineer Biomaterials for Neural Stem Cell Culture." , 32:1484-1494 (PMID 21129772). View PDF
Lim, K-I., R.R. Klimczak, J.H. Yu, and D.V. Schaffer (2010) "Specific Insertions of Zinc Finger Domains into Gag-Pol Yields Engineered Retroviral Vectors with Selective Integration Properties." , 107:12475-12480 (PMID 20616052). View PDF
Recent Selected Publications
Willerth, S.M., H.A.M. Pedro, L. Pachter, L.M. Humeau, A.P. Arkin, and D.V. Schaffer (2010) "Development of a Low Bias Method for Characterizing Viral Populations Using Next Generation Sequencing Technology." 5:e13564 (PMID 21042592). View PDF
Kurpinski, K., H. Lam, J. Chu, A. Kim, E. Tsay, S. Agrawal, D.V. Schaffer, A. Winoto, and S. Li (2010) "Transforming Growth Factor-beta and Notch Signaling Mediate Stem Cell Differentiation into Smooth Muscle Cells." , 4:734-42 (PMID 20146266).
Aviran, S., P.S. Shah, D.V. Schaffer, and A.P. Arkin (2010) "Computational Models of HIV-1 Resistance to Gene Therapy Elucidate Therapy Design Principles." , 6:e1000883 (PMID 20711350). View PDF
Burnett, J.C., K.I. Lim, A. Calafi, J.J. Rossi, D.V. Schaffer (co-corresponding author), A.P. Arkin (2010) "Combinatorial Latency Reactivation for HIV-1 Subtypes and Variants." , 84:5958-5974 (PMID 20357084). View PDF
Peltier, J., B.K. Ormerod, and D.V. Schaffer (2010) "Isolation of Adult Hippocampal Neural Progenitors." , 621:57-63 (PMID 20405359). View PDF
Peltier, J., S. Agrawal, M.J. Robertson, and D.V. Schaffer (2010) "In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors." , 621:65-87 (PMID 20405360). View PDF
Saha, K., J. Kim, E. Irwin, J. Yoon, F. Momin, V. Trujillo, D.V. Schaffer, K.E. Healy, and R.C. Hayward "Surface Creasing Instability of Soft Polyacrylamide Cell Culture Substrates." , 99:L94-96 (PMID 21156124). View PDF
Keung, A.J., K.E. Healy, S. Kumar, and D.V. Schaffer (2010) "Biophysics and Dynamics of Natural and Engineered Stem Cell Microenvironments." , 2:49-64 (PMID 20836010). View PDF
Robertson, M.J., J. Peltier, and D.V. Schaffer (2010) "In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors." , 621:89-102 (PMID 20405361). View PDF
Shah, P.S. and D.V. Schaffer (2010) "Gene Therapy Takes a Cue from HAART: Combinatorial Antiviral Therapeutics Reach the Clinic." , 2:36ps30 (PMID 20555021). View PDF
Skupsky, R., J.C. Burnett, J.E. Foley, D.V. Schaffer (co-corresponding author), and A.P. Arkin (2010) "HIV Promoter Integration Site Primarily Modulates Transcriptional Burst Size Rather Than Frequency." , 6:e1000952 (PMID 20711350). View PDF
J.T. Koerber, R. Klimczak, J-H. Jang, D. Dalkara, J.G. Flannery, and D.V. Schaffer (2009) "Molecular Evolution of Adeno-Associated Virus for Enhanced Glial Gene Delivery." Molecular Therapy, 17: 2088-2095 (PMID 19672246). View PDF
Excoffon, K.J., J.T. Koerber, D.D. Dickey, M. Murtha, S. Keshavjee, B.K. Kaspar, J. Zabner, and D.V. Schaffer (2009) "Directed Evolution of Adeno-Associated Virus to an Infectious Respiratory Virus." Proceedings of the National Academy of Sciences USA, 106:3865-3870 (PMID 19237554). View PDF
Recent Selected Publications
Dalkara, D., K.D. Kolstad, N. Caporale, M. Visel, R.R. Klimczak, D.V. Schaffer, and J.G. Flannery (2009) "Inner Limiting Membrane Barriers to AAV Mediated Retinal Transduction from the Vitreous." Molecular Therapy, 17:2096-2102 (PMID 19672248). View PDF
Agrawal, S., C. Archer, and D.V. Schaffer "Computational Models of the Notch Network Elucidate Mechanisms of Context-dependent Signaling." Public Library of Science (PLoS) Computational Biology (in press). View PDF
Banerjeea, A., M. Arhaa, S. Choudhary, R.S. Ashton, S.R. Bhatia, D.V. Schaffer, and R.S. Kane (2009) "The Influence of Hydrogel Modulus on the Proliferation and Differentiation of Encapsulated Neural Stem Cells." Biomaterials, 30:4695-9 (PMID 19539367). View PDF
Burnett, J.C., K. Miller-Jensen, P.S. Shah, A.P. Arkin, and D.V. Schaffer (2009) "Control of Stochastic Gene Expression by Host Factors at the HIV Promoter." Public Library of Science (PLoS) Pathogens, 5(1) (PMID 19132086). View PDF
Meng, Y., S. Eshghi, Y. Li, R. Schmidt, D.V. Schaffer, and K.E. Healy (2009) "Characterization of Integrin Engagement during Defined Human Embryonic Stem Cell Culture." FASEB Journal, 24:0 (PMID 19933311). View PDF
R. Klimczak, J.T. Koerber, D. Dalkara, J.G. Flannery, and D.V. Schaffer (2009) "A Novel Adeno-Associated Viral Variant for Efficient and Selective Intravitreal Transduction of Rat MÜller Cells." Public Library of Science (PLoS) ONE, 4:e7467 (PMID 19826483). View PDF
Carlson, M.E., M.J. Conboy, M. Hsu, L. Barchas, J. Jeong, A. Agrawal, A.J. Mikels, S. Agrawal, D.V. Schaffer, and I.M. Conboy (2009) "Relative Roles of TGF-beta1 and Wnt in the Systemic Regulation and Aging of Satellite Cell Responses." Aging Cell, 8:676-689 (PMID 19732043).
Kwon, I. and D.V. Schaffer (2008) "Designer Gene Delivery Vectors: Molecular Engineering and Evolution of Adeno-Associated Viral Vectors for Enhanced Gene Transfer." Pharmaceutical Research, 25:489-499. View PDF
Koerber, J.T. and D.V. Schaffer "Transposon-Based Mutagenesis Generates Diverse Adeno-Associated Viral Libraries with Novel Gene Delivery Properties." Methods in Molecular Biology, 434:161-170 (PMID 18470644). View PDF
Koerber, J.T., J-H. Jang, and D.V. Schaffer "DNA Shuffling of Adeno-Associated Virus Yields Functionally Diverse Viral Progeny." Molecular Therapy, 16:1703-9. (PMID 18728640). View PDF
Ricci, C., V. Pastukh, J. Leonard, J. Turrens, G. Wilson, D. Schaffer, and S.W. Schaffer(2008) "Mitochondrial DNA Damage Triggers Mitochondrial Superoxide Generation and Apoptosis." American Journal of Physiology, 294:C413-22 PMID 18077603).
Leonard, J.N., P.S. Shah, J.C. Burnett, and D.V. Schaffer (2008) "HIV Evades RNA Interference Directed at TAR by an Indirect Compensatory Mechanism." Cell Host and Microbe, 4:484-94 (PMID 18996348). Selected as the Featured Article. View PDF
Lim, K-I., and D.V. Schaffer (2008) "Library Selection Approaches to Engineering Enhanced Retroviral and Lentiviral Vectors." Combinatorial Chemistry and High Throughput Screening, 11:111-117 (PMID 18336204). View PDF
Schaffer, D.V. (corresponding author), J.T. Koerber, and K.-I. Lim (2008) "Molecular Engineering of Viral Gene Delivery Vehicles." Annual Reviews in Biomedical Engineering, 10:169-94 (PMID 18647114). View PDF
Robertson, M.J., P. Gip, and D.V. Schaffer (2008) "Neural Stem Cell Engineering: Directed Differentiation of Adult and Embryonic Stem Cells into Neurons." Frontiers in Bioscience, 13:21-50 (PMID 17981526). View PDF
Eshghi, S. and D.V. Schaffer "Engineering Microenvironments to Control Stem Cell Fate and Function." Stembook Stembook, D. Melton, ed. View PDF
Little, L., K.E. Healy, and D.V. Schaffer (2008) "Engineering Biomaterials for Synthetic Neural Stem Cell Microenvironments." Chemical Reviews, 108:1787-1796 (PMID 18476674). View PDF
Wall, S., K. Saha, R. Aston, K. Kam, D.V. Schaffer, and K.E. Healy "Multivalency of Sonic Hedgehog Conjugated to Linear Polymer Chains Modulates Protein Potency." Bioconjugate Chemistry, 19:806-812 (PMID 18380472). View PDF
Saha, K., A.J. Keung, E.F. Irwin, Y. Li, D.V. Schaffer (co-corresponding author), and K.E. Healy "Substrate Modulus Directs Neural Stem Cell Behavior." Biophysical Journal, 95:4426-38. (PMID 18658232). View PDF
Ashton, R.S., A. Banerjee, S. Punyani, D.V. Schaffer, and R.S Kane (2007) "Scaffolds Based on Degradable Alginate Hydrogels and Poly(lactide-co-glycolide) Microspheres for Stem Cell Culture Biomaterials." Biomaterials, 28:5518-5525. View PDF
Peltier, J., A. O'Neill, and D.V. Schaffer (2007) "PI3K/Akt and CREB Regulate Adult Neural Hippocampal Progenitor Proliferation and Differentiation." Developmental Neurobiology, 67:1348-1361. View PDF
Jang, J-H., K-I. Lim, and D.V. Schaffer (2007) "Library Selection and Directed Evolution Approaches to Engineering Targeted Viral Vectors." Biotechnology & Bioengineering, 98:515-524. View PDF
Leonard, J.N., P. Ferstl, A. Delgado, and D.V. Schaffer (2007) "Enhanced Preparation of Adeno-Associated Viral Vectors by Using High Hydrostatic Pressure to Selectively Inactivate Helper Adenovirus." Biotechnology & Bioengineering, 97:1170-1179. Selected as a Journal Spotlight Article. View PDF
Saha, K., J. Pollock, D.V. Schaffer (co-corresponding author), and K.E. Healy (2007) "Designing Synthetic Materials to Control Stem Cell Phenotype." Current Opinion in Chemical Biology, 11:381-387. View PDF
Koerber, J.T., J-H. Jang, and D.V. Schaffer (2007) "Engineering Adeno-Associated Virus for One Step Purification via Immobilized Metal Affinity Chromatography." Human Gene Therapy, 18:367-378. View PDF
Ho, J.E., E.H. Chung, S. Wall, D.V. Schaffer, and K.E. Healy (2007) "Immobilized Sonic Hedgehog N-Terminal Signaling Domain Enhances Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells." Journal of Biomedical Materials Research Part A, 83A:1200-1208. View PDF
Saha, K., E.F. Irwin, J. Kozhukh, D.V. Schaffer (corresponding author), and K.E. Healy (corresponding author) (2007) "Biomimetic Interfacial Interpenetrating Polymer Networks Control Neural Stem Cell Behavior." Journal of Biomedical Materials Research Part A, 81:240-249. View PDF
Ashton, R.S., J. Peltier, C.A. Fasano, A. O'Neill, J. Leonard, S. Temple, D.V. Schaffer (co-corresponding author), and R.S. Kane (2007) "High-Throughput Screening of Gene Function in Stem Cells Using Clonal Microarrays." Stem Cells, 25:2928-2935. View PDF
Leonard, J. and D.V. Schaffer (2006) "Antiviral RNAi Strategies: Emerging Approaches for Hitting a Moving Target." Gene Therapy, 13:532-540. View PDF
Saha, K. and D.V. Schaffer (2006) "Signaling Dynamics in Sonic Hedgehog Tissue Patterning." Development, 133:889-900. View PDF
Koerber, J.T., N. Maheshri, B.K. Kaspar, and D.V. Schaffer (2006) "Construction of Diverse Adeno-Associated Viral Libraries for Directed Evolution of Enhanced Gene Delivery Vehicles." Nature Protocols, 1:701-706. View PDF
Recent Selected Publications
Greenberg, K.P., S.F. Geller, D.V. Schaffer, and J.G. Flannery (2006) "Targeted Transgene Expression in Muller Glia of Normal and Diseased Retinas Using Lentiviral Vectors." Investigative Ophthalmology & Visual Science, 48:1844-1852. View PDF
Maheshri, N., J.T. Koerber, B. Kaspar, and D.V. Schaffer (2006) "Directed Evolution of Adeno-Associated Virus Yields Enhanced Gene Delivery Vectors." Nature Biotechnology, 24:198-204. View PDF
Recent Selected Publications
Greenberg K.P., E.S. Lee, D.V. Schaffer, and J.G. Flannery (2006) "Gene Delivery to the Retina Using Lentiviral Vectors." Advances in Experimental Medicine and Biology, 572: 255-266. View PDF
Abranches, E., O'Neill, A., Robertson, M.J., Schaffer, D.V. (corresponding author), and J. Cabral (2006) "Development of Quantitative PCR Methods to Analyze Adult Neural Progenitor Cell Culture State." Biotechnology & Applied Biochemistry, 44:1-8. View PDF
Yu, J.H. and D.V. Schaffer (2006) "High-Throughput, Library-Based Selection of a Murine Leukemia Virus to Infect Nondividing Cells." Journal of Virology, 80:8981-8988. Selected as a Journal of Virology Spotlight Article. View PDF
Jang, J-H. and D.V. Schaffer (2006) "Microarraying the Cellular Microenvironment" Molecular Systems Biology, 2:39. View PDF
Yu, J. and D.V. Schaffer (2006) "Selection of Novel Vesicular Stomatitis Virus Glycoprotein Variants from a Peptide Insertion Library for Enhanced Purification of Retroviral and Lentiviral Vectors." Journal of Virology, 80:3285-3292. View PDF
Leonard, J. and D.V. Schaffer (2005) "Computational Design of RNA Interference Strategies that Resist HIV Escape." Journal of Virology, 79:1645-1654. View PDF
Agrawal, S. and D.V. Schaffer (2005) "In Situ Stem Cell Therapy: Novel Targets, Familiar Challenges." Trends in Biotechnology, 23:78-83. View PDF
Schaffer, D.V. and W.C. Zhou (2005) "Gene Therapy and Gene Delivery Systems as Future Human Therapeutics." Advances in Biochemical Engineering & Biotechnology, 99:1-5.
Yu, J. and D.V. Schaffer (2005) "Advanced Targeting Strategies for Murine Retroviral and Adeno-Associated Viral Vectors." Advances in Biochemical Engineering & Biotechnology, 99:147-167. View PDF
Lee, G., Maheshri, N., Kaspar, B.K., and D.V. Schaffer (2005) "PEG Conjugation Moderately Protects Adeno-Associated Viral Vectors Against Antibody Neutralization." Biotechnology & Bioengineering, 92:24-34. View PDF
Weinberger, L.S., J.C. Burnett, J.E. Toettcher, A.P. Arkin, and D.V. Schaffer (2005) "Stochastic Gene Expression in a Lentiviral Feedback Loop: HIV-1 Tat Expression Drives Phenotypic Diversity." Cell, 122:169-182. View PDF
Recent Selected Publications
Lai, K., M.J. Robertson, and D.V. Schaffer (2004) "The Sonic Hedgehog Signaling System as a Bistable Genetic Switch." Biophysical Journal, 86:2748-2757. View PDF
O'Neill, A. and D.V. Schaffer (2004) "The Biology and Engineering of Stem Cell Control." Biotechnology & Applied Biochemistry, 40:5-16. View PDF
Ignowski, J. and D.V. Schaffer (2004) "Kinetic Analysis and Modeling of Firefly Luciferase as a Quantitative Reporter Gene in Live Mammalian Cells." Biotechnology & Bioengineering, 86(7):827-34. View PDF
Schaffer, D.V. and F.H. Gage (2004) "Neurogenesis and Neuroadaptation" Neuromolecular Medicine, 5:1-9. View PDF
Maheshri, N. and D.V. Schaffer (2003) "Computational and Experimental Analysis of DNA Shuffling." Proceedings of the National Academy of Sciences USA, 100:3071-3076. View PDF
Lai, K, B.K. Kaspar, F.H. Gage, and D.V. Schaffer (2003) "Sonic Hedgehog Regulates Adult Neural Progenitor Proliferation in Vitro and in Vivo." Nature Neuroscience, 6(1):21-27. View PDF
Weinberger, L., D.V. Schaffer (corresponding author), and A.P. Arkin (corresponding author) (2003) "Theoretical Design of a Gene Therapy to Prevent AIDS but Not HIV-1 Infection." Journal of Virology, 77:10028-10036. View PDF
Kaspar, B.K., D. Erickson, D. Schaffer, L. Hinh, F.H. Gage, and D.A. Peterson (2002) "Targeted Retrograde Gene Delivery for Neuronal Protection." Molecular Therapy, 5:50-6. View PDF
Wiewrodt, W., A.P. Thomas, L. Cipelletti, D.A. Weitz, S.I. Feinstein, D.V. Schaffer, S.M. Albelda, M. Koval, and V.R. Muzykantov (2002) "Size-Dependent Intracellular Immunotargeting into Endothelial Cells via a Surface Adhesion Molecule, PECAM-1," Blood, 99:912-22. View PDF
Kaspar, B.K., Vissel, B., Bengoechea, T., Crone, S., Randolph-Moore, L., Muller, R., Brandon,E.P., Schaffer, D., Verma, I.M., Lee, K.F., Heinemann, S.F., and F.H. Gage (2002) "Adeno-associated Virus Effectively Mediates Conditional Gene Modification in the Brain." Proceedings of the National Academy of Sciences USA, 99:2320-5
Mozaffari, M.S. and D.V. Schaffer (2001) "Taurine Modulates Arginine Vasopressin-Mediated Regulation of Renal Function." Journal of Cardiovascular Pharmacology, 37(6): 742-750.
Schaffer, D.V. and D.A. Lauffenburger (2000) "Targeted Synthetic Gene Delivery Vectors." Current Opinions in Molecular Therapeutics, 2: 155-161. View PDF
Lauffenburger, D.A. and D.V. Schaffer (1999) "The Matrix Delivers." Nature Medicine, 5: 733-734. View PDF
Schaffer, D.V., N. Fidelman, N. Dan, and D.A. Lauffenburger (1999) "Vector Unpackaging as a Potential Barrier for Receptor-Mediated Polyplex Gene Delivery." Biotechnology & Bioengineering, 67:598-606. View PDF
Schaffer, D.V. and D.A. Lauffenburger (1998) "Optimization of Cell Surface Binding Enhances Efficiency and Specificity of Molecular Conjugate Gene Delivery." Journal of Biological Chemistry, 273:28004-28009. View PDF
Schaffer, D.V., R.L. Neve, and D.A. Lauffenburger (1996) "Use of Green Fluorescent Protein as a Reporter of Epidermal Growth Factor Receptor-Mediated Gene Delivery." Tissue Engineering, 3:53-63.
Gramer, M.J., D.V. Schaffer, M.B. Sliwkowski, and C.F. Goochee (1994) "Purification and Purification and Characterization of an a-L-Fucosidase from Chinese Hamster Ovary Cell Culture Supernatant." Glycobiology, 4:611-616.
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